ACTN2
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell adhesion [TAS]
- focal adhesion assembly [IMP]
- microspike assembly [IDA]
- muscle filament sliding [TAS]
- negative regulation of potassium ion transmembrane transporter activity [IMP]
- negative regulation of potassium ion transport [IMP]
- negative regulation of protein localization to cell surface [IMP]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of potassium ion transmembrane transporter activity [IDA]
- positive regulation of potassium ion transport [IDA]
- protein homotetramerization [IDA]
- regulation of apoptotic process [NAS]
- regulation of membrane potential [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KCNA5
Gene Ontology Biological Process
- atrial cardiac muscle cell action potential [IMP]
- membrane hyperpolarization [IMP]
- potassium ion export [IDA, IMP]
- potassium ion transport [IDA]
- regulation of atrial cardiac muscle cell membrane repolarization [IMP]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of insulin secretion [TAS]
- regulation of membrane potential [IDA]
- regulation of potassium ion transport [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- alpha-actinin binding [IPI]
- delayed rectifier potassium channel activity [IDA, IMP]
- outward rectifier potassium channel activity [IDA, IMP]
- protein binding [IPI]
- protein kinase binding [IPI]
- scaffold protein binding [IPI]
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization [IMP]
- alpha-actinin binding [IPI]
- delayed rectifier potassium channel activity [IDA, IMP]
- outward rectifier potassium channel activity [IDA, IMP]
- protein binding [IPI]
- protein kinase binding [IPI]
- scaffold protein binding [IPI]
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization [IMP]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
alpha-actinin-2 couples to cardiac Kv1.5 channels, regulating current density and channel localization in HEK cells.
Voltage-gated K(+) (Kv) channels are particularly important in the physiology of excitable cells in the heart and the brain. PSD-95 is known to cluster Shaker channels and NMDA receptors and the latter is known to couple through alpha-actinin-2 to the post-synaptic cytoskeleton [Wyszynski et al. (1997) Nature 385, 439-442], but the mechanisms by which Kv channels are linked to the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ACTN2 KCNA5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KCNA5 ACTN2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KCNA5 ACTN2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| ACTN2 KCNA5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| KCNA5 ACTN2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| ACTN2 KCNA5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID