Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

LncRNA SAMD12-AS1 promotes cell proliferation and inhibits apoptosis by interacting with NPM1.

Liu Q, Liu N, Shangguan Q, Zhang F, Chai W, Tong X, Zhao X, Li Z, Qi D, Ye X

Chronic hepatitis B virus infection is a major risk factor for hepatocellular carcinoma. HBV infection affects lncRNA expression in infected cells, but the detailed mechanism and biological significance are not yet clear. In this study, we focused on exploring the function of the HBV-upregulated lncRNA SAMD12-AS1 in cell proliferation. We found that there is a higher level of SAMD12-AS1 expression ... [more]

Sci Rep Dec. 12, 2018; 9(1);11593 [Pubmed: 31406141]

Throughput

  • High Throughput

Additional Notes

  • assayed using RIP (RNA immunoprecipitation) with an RNA derived from the bait gene SAMD12, with associated hit proteins identified by mass spectrometry

Curated By

  • BioGRID