BAIT

FBXL17

FBXO13, Fbl17, Fbx13
F-box and leucine-rich repeat protein 17
UPS Project
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

CSNK2B

CK2B, CK2N, CSK2B, G5A, DADB-127H9.2
casein kinase 2, beta polypeptide
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation.

Mason B, Flach S, Teixeira FR, Manzano Garcia R, Rueda OM, Abraham JE, Caldas C, Edwards PAW, Laman H

In cancer, many genes are mutated by genome rearrangement, but our understanding of the functional consequences of this remains rudimentary. Here we report the F-box protein encoded by FBXL17 is disrupted in the region of the gene that encodes its substrate-binding leucine rich repeat (LRR) domain. Truncating Fbxl17 LRRs impaired its association with the other SCF holoenzyme subunits Skp1, Cul1 ... [more]

Cell. Mol. Life Sci. Jul. 01, 2020; 77(13);2605-2620 [Pubmed: 31560077]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FBXL17 CSNK2B
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low/High-BioGRID
2757885

Curated By

  • BioGRID