Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.

Biancospino M, Buel GR, Nino CA, Maspero E, Scotto di Perrotolo R, Raimondi A, Redlingshoefer L, Weber J, Brodsky FM, Walters KJ, Polo S

Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively ... [more]

Nat Commun Dec. 31, 2018; 10(1);4974 [Pubmed: 31672988]

Throughput

  • Low Throughput

Additional Notes

  • Source of Hip1R not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLTA HIP1R
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3351728
CLTA HIP1R
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High110BioGRID
2983496
HIP1R CLTA
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
2758855

Curated By

  • BioGRID