BAIT

PRMT2

HRMT1L1
protein arginine methyltransferase 2
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of protein arginine methyltransferase 8 (PRMT8) activity by its N-terminal domain.

Sayegh J, Webb K, Cheng D, Bedford MT, Clarke SG

Human protein arginine methyltransferase PRMT8 has been recently described as a type I enzyme in brain that is localized to the plasma membrane by N-terminal myristoylation. The amino acid sequence of human PRMT8 is almost 80% identical to human PRMT1, the major protein arginine methyltransferase activity in mammalian cells. However, the activity of a recombinant PRMT8 GST fusion protein toward ... [more]

J. Biol. Chem. Dec. 14, 2007; 282(50);36444-53 [Pubmed: 17925405]

Throughput

  • Low Throughput

Curated By

  • BioGRID