BAIT

CHRNE

ACHRE, CMS1D, CMS1E, CMS2A, FCCMS, SCCMS
cholinergic receptor, nicotinic, epsilon (muscle)
Homo sapiens
PREY

CHRNA1

ACHRA, ACHRD, CHRNA, CMS2A, FCCMS, SCCMS
cholinergic receptor, nicotinic, alpha 1 (muscle)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Extracellular domains mediating epsilon subunit interactions of muscle acetylcholine receptor.

Yu XM, Hall ZW

Ligand-gated ion channels, a major class of cell-surface proteins, have a pseudosymmetric structure with five highly homologous subunits arranged around a central ion pore. The correct assembly of each channel, whose subunit composition varies with cell type and stage of development, requires specific recognition between the subunits. Assembly of the pentameric form of the acetylcholine receptor from adult muscle (AChR; ... [more]

Nature Jul. 04, 1991; 352(6330);64-7 [Pubmed: 1712080]

Throughput

  • Low Throughput

Curated By

  • BioGRID