An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115.

Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G

p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex ... [more]

J. Cell Biol. Apr. 01, 2002; 157(1);45-62 [Pubmed: 11927603]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GOSR1 USO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.9443	BioGRID	3270267
USO1 GOSR1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Shorter J (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

USO1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein transporter activity [IBA]

Gene Ontology Cellular Component

GOSR1