BAIT

IKBKG

AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
GO Process (32)
GO Function (8)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens
PREY

COPS3

CSN3, SGN3
COP9 signalosome subunit 3
GO Process (4)
GO Function (1)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CSN3 interacts with IKKgamma and inhibits TNF- but not IL-1-induced NF-kappaB activation.

Hong X, Xu L, Li X, Zhai Z, Shu H

The transcription factor nuclear factor kappaB (NF-kappaB) plays a pivotal role in immune and inflammatory responses. Activation of NF-kappaB requires the activity of IKK, a kinase complex that contains two catalytic subunits, IKKalpha and IKKbeta, and a regulatory subunit IKKgamma. To understand how IKK activity is regulated, we searched for IKKgamma-interacting proteins by the yeast two-hybrid system. These screenings identified ... [more]

FEBS Lett. Jun. 15, 2001; 499(1);133-6 [Pubmed: 11418127]

Throughput

  • Low Throughput

Curated By

  • BioGRID