BAIT

ITGB1

CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, RP11-479G22.2
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
GO Process (25)
GO Function (6)
GO Component (21)
Homo sapiens
PREY

SLC9A5

NHE5
solute carrier family 9, subfamily A (NHE5, cation proton antiporter 5), member 5
GO Process (4)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RACK1 associates with NHE5 in focal adhesions and positively regulates the transporter activity.

Onishi I, Lin PJ, Diering GH, Williams WP, Numata M

Na+/H+ exchanger isoform 5 (NHE5) is a brain-enriched NHE that may play important roles in ion homeostasis and cell-volume regulation. However, the regulation mechanism of NHE5 has not been fully elucidated. Here, we show that Receptor for Activated C-kinase 1 (RACK1) directly binds to NHE5 and positively regulates the transporter function. NHE5 co-localized with RACK1 as well as beta1 integrin, ... [more]

Cell. Signal. Jan. 01, 2007; 19(1);194-203 [Pubmed: 16920332]

Throughput

  • Low Throughput

Curated By

  • BioGRID