BAIT

GRIN2A

GluN2A, NMDAR2A, NR2A
glutamate receptor, ionotropic, N-methyl D-aspartate 2A
GO Process (62)
GO Function (19)
GO Component (13)

Gene Ontology Biological Process

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NMDA di-heteromeric receptor populations and associated proteins in rat hippocampus.

Al-Hallaq RA, Conrads TP, Veenstra TD, Wenthold RJ

Subunit composition of NMDA receptors (NMDARs) determines a range of physiological properties, downstream signaling effects, and binding partners. Differential localization of NR2A- or NR2B-containing NMDARs within the neuron and subunit-specific protein associations may explain differences in NR2A and NR2B contributions to synaptic plasticity and excitotoxic cell death. This question is complicated by the existence of tri-heteromeric complexes (NR1/NR2A/NR2B). To date, ... [more]

J. Neurosci. Aug. 01, 2007; 27(31);8334-43 [Pubmed: 17670980]

Throughput

  • Low Throughput

Curated By

  • BioGRID