EPHB1
Gene Ontology Biological Process
- angiogenesis [IDA]
- axon guidance [ISS]
- cell chemotaxis [IDA]
- cell-substrate adhesion [IDA]
- central nervous system projection neuron axonogenesis [ISS]
- dendritic spine development [ISS]
- dendritic spine morphogenesis [ISS]
- detection of temperature stimulus involved in sensory perception of pain [ISS]
- ephrin receptor signaling pathway [IDA]
- establishment of cell polarity [ISS]
- neural precursor cell proliferation [ISS]
- neurogenesis [ISS]
- positive regulation of synapse assembly [ISS]
- protein autophosphorylation [IDA]
- regulation of ERK1 and ERK2 cascade [IDA]
- regulation of JNK cascade [IDA]
- regulation of neuron death [IMP]
- retinal ganglion cell axon guidance [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACP1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Eph receptors discriminate specific ligand oligomers to determine alternative signaling complexes, attachment, and assembly responses.
Eph family receptor tyrosine kinases (including EphA3, EphB4) direct pathfinding of neurons within migratory fields of cells expressing gradients of their membrane-bound ligands. Others (EphB1 and EphA2) direct vascular network assembly, affecting endothelial migration, capillary morphogenesis, and angiogenesis. To explore how ephrins could provide positional labels for cell targeting, we tested whether endogenous endothelial and P19 cell EphB1 (ELK) and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EPHB1 ACP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID