Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m6A reader YTHDF3.

Ni W, Yao S, Zhou Y, Liu Y, Huang P, Zhou A, Liu J, Che L, Li J

YAP activation is crucial for cancer development including colorectal cancer (CRC). Nevertheless, it remains unclear whether N6-Methyladenosine (m6A) modified transcripts of long noncoding RNAs (lncRNAs) can regulate YAP activation in cancer progression. We investigated the functional link between lncRNAs and the m6A modification in YAP signaling and CRC progression.YAP interacting lncRNAs were screened by RIP-sequencing, RNA FISH and immunofluorescence co-staining ... [more]

Mol. Cancer Dec. 16, 2018; 18(1);143 [Pubmed: 31619268]

Throughput

  • High Throughput

Additional Notes

  • bait protein YAP was IP'd (immunoprecipitated) and associated hit RNAs were identified, possibly by qRT-PCR (quantitative reverse transcription polymerase chain reaction)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GAS5 YAP1
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Low-BioGRID
2766562

Curated By

  • BioGRID