BAIT

GNAO1

EIEE17, G-ALPHA-o, GNAO
guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Homo sapiens
PREY

CACNA1B

BIII, CACNL1A5, CACNN, Cav2.2
calcium channel, voltage-dependent, N type, alpha 1B subunit
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Binding of G alpha(o) N terminus is responsible for the voltage-resistant inhibition of alpha(1A) (P/Q-type, Ca(v)2.1) Ca(2+) channels.

Kinoshita M, Nukada T, Asano T, Mori Y, Akaike A, Satoh M, Kaneko S

G-protein-mediated inhibition of presynaptic voltage-dependent Ca(2+) channels is comprised of voltage-dependent and -resistant components. The former is caused by a direct interaction of Ca(2+) channel alpha(1) subunits with G beta gamma, whereas the latter has not been characterized well. Here, we show that the N terminus of G alpha(o) is critical for the interaction with the C terminus of the ... [more]

J. Biol. Chem. Aug. 03, 2001; 276(31);28731-8 [Pubmed: 11395521]

Throughput

  • Low Throughput

Curated By

  • BioGRID