An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The liver-specific human alpha(1)-microglobulin/bikunin precursor (AMBP) is capable of self-association.

Tyagi S, Salier JP, Lal SK

alpha-1-Microglobulin (A1M) and bikunin are two plasma glycoproteins encoded by an alpha-1-microglobulin/bikunin precursor (AMBP) gene. Despite their lack of any structural or functional relationship, both A1M and bikunin originate from AMBP cleavage by a furin-like protease that releases the two mature molecules. The AMBP gene maintains a tight control over its expression by a unique enhancer, which is controlled by ... [more]

Arch. Biochem. Biophys. Mar. 01, 2002; 399(1);66-72 [Pubmed: 11883904]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AMBP AMBP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Berggard T (1997)	Low	-	BioGRID	-
AMBP AMBP	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Tyagi S (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AMBP

Gene Ontology Biological Process

Gene Ontology Molecular Function

IgA binding [IDA]calcium channel inhibitor activity [NAS]calcium oxalate binding [NAS]heme binding [IDA]protein binding [IPI]protein homodimerization activity [IPI]serine-type endopeptidase inhibitor activity [TAS]

Gene Ontology Cellular Component