CAMK2A
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO, ISS]
- calcium ion transport [ISO, ISS]
- ionotropic glutamate receptor signaling pathway [IDA]
- peptidyl-serine phosphorylation [IDA, ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of calcium ion transport [ISO, ISS]
- positive regulation of cardiac muscle cell apoptotic process [ISO, ISS]
- protein autophosphorylation [IDA, IMP, ISO]
- protein phosphorylation [IDA, IMP, ISO]
- regulation of mitochondrial membrane permeability involved in apoptotic process [ISO, ISS]
- regulation of neuronal synaptic plasticity [ISO, ISS]
- regulation of neurotransmitter secretion [ISO, ISS]
- response to ischemia [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACTN2
Gene Ontology Biological Process
- focal adhesion assembly [ISO]
- microspike assembly [ISO]
- muscle contraction [ISO]
- negative regulation of potassium ion transmembrane transporter activity [ISO]
- negative regulation of potassium ion transport [ISO]
- negative regulation of protein localization to cell surface [ISO]
- positive regulation of potassium ion transmembrane transporter activity [ISO]
- positive regulation of potassium ion transport [ISO]
- positive regulation of receptor activity [ISO]
- protein homotetramerization [ISO]
- regulation of membrane potential [ISO]
- regulation of nucleic acid-templated transcription [ISO]
Gene Ontology Molecular Function- FATZ binding [ISO]
- LIM domain binding [ISO]
- actin filament binding [ISO]
- cytoskeletal protein binding [ISO]
- identical protein binding [ISO]
- ion channel binding [ISO]
- ligand-dependent nuclear receptor transcription coactivator activity [ISO]
- protein binding [IPI]
- protein dimerization activity [ISO]
- thyroid hormone receptor coactivator activity [ISO]
- titin Z domain binding [ISO]
- titin binding [ISO]
- FATZ binding [ISO]
- LIM domain binding [ISO]
- actin filament binding [ISO]
- cytoskeletal protein binding [ISO]
- identical protein binding [ISO]
- ion channel binding [ISO]
- ligand-dependent nuclear receptor transcription coactivator activity [ISO]
- protein binding [IPI]
- protein dimerization activity [ISO]
- thyroid hormone receptor coactivator activity [ISO]
- titin Z domain binding [ISO]
- titin binding [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2.
Dendritic calcium/calmodulin-dependent protein kinase II (CaMKII) is dynamically targeted to the synapse. We show that CaMKIIalpha is associated with the CaMKII-binding proteins densin-180, the N-methyl-D-aspartate receptor NR2B subunit, and alpha-actinin in postsynaptic density-enriched rat brain fractions. Residues 819-894 within the C-terminal domain of alpha-actinin-2 constitute the minimal CaMKII-binding domain. Similar amounts of Thr286-autophosphorylated CaMKIIalpha holoenzyme [P-T286]CaMKII bind to alpha-actinin-2 as ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ACTN2 CAMK2A | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID