SYNJ1
Gene Ontology Biological Process
- inositol phosphate dephosphorylation [ISS]
- inositol phosphate metabolic process [TAS]
- neurotransmitter transport [ISS]
- phosphate-containing compound metabolic process [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol dephosphorylation [ISS]
- phosphatidylinositol metabolic process [ISS]
- phospholipid metabolic process [TAS]
- positive regulation of synaptic vesicle uncoating [TAS]
- regulation of synaptic vesicle membrane organization [TAS]
- small molecule metabolic process [TAS]
- synaptic vesicle endocytosis [ISS]
- synaptic vesicle priming [ISS]
- synaptic vesicle transport [ISS]
- synaptic vesicle uncoating [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AMPH
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Synaptojanin forms two separate complexes in the nerve terminal. Interactions with endophilin and amphiphysin.
Endophilin is a recently discovered src homology 3 domain-containing protein that is a major in vitro binding partner for synaptojanin. To further characterize endophilin, we generated an antipeptide antibody. Endophilin is enriched in the brain, and immunofluorescence analysis reveals a high concentration of the protein in synaptic terminals, where it colocalizes with synaptojanin. In vitro binding assays demonstrate that endophilin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AMPH SYNJ1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SYNJ1 AMPH | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID