BAIT

EFNA2

ELF-1, EPLG6, HEK7-L, LERK-6, LERK6
ephrin-A2
GO Process (5)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ADAM10

AD10, AD18, CD156c, HsT18717, MADM, RAK, kuz
ADAM metallopeptidase domain 10
GO Process (20)
GO Function (8)
GO Component (13)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulated cleavage of a contact-mediated axon repellent.

Hattori M, Osterfield M, Flanagan JG

Contact-mediated axon repulsion by ephrins raises an unresolved question: these cell surface ligands form a high-affinity multivalent complex with their receptors present on axons, yet rather than being bound, axons can be rapidly repelled. We show here that ephrin-A2 forms a stable complex with the metalloprotease Kuzbanian, involving interactions outside the cleavage region and the protease domain. Eph receptor binding ... [more]

Science Aug. 25, 2000; 289(5483);1360-5 [Pubmed: 10958785]

Throughput

  • Low Throughput

Curated By

  • BioGRID