BAIT

TUB4

gamma-tubulin, L000002751, YLR212C
Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

GIM4

PFD2, L000004368, YEL003W
Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Interrogation of γ-tubulin alleles using high-resolution fitness measurements reveals a distinct cytoplasmic function in spindle alignment.

Shulist K, Yen E, Kaitna S, Leary A, Decterov A, Gupta D, Vogel J

γ-Tubulin has a well-established role in nucleating the assembly of microtubules, yet how phosphorylation regulates its activity remains unclear. Here, we use a time-resolved, fitness-based SGA approach to compare two γ-tubulin alleles, and find that the genetic interaction profile of γtub-Y362E is enriched in spindle positioning and cell polarity genes relative to that of γtub-Y445D, which is enriched in genes ... [more]

Sci Rep Dec. 12, 2016; 7(1);11398 [Pubmed: 28900268]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: fitness (APO:0000216)
  • phenotype: colony size (APO:0000063)
  • phenotype: vegetative growth (APO:0000106)
  • phenotype: fitness (APO:0000216)
  • phenotype: colony size (APO:0000063)

Additional Notes

  • SGI determined using GAMER
  • gammatub-Y362E
  • gammatub-Y445D

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GIM4 TUB4
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
158098

Curated By

  • BioGRID