Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
Long non-coding RNA KRT19P3 suppresses proliferation and metastasis through COPS7A-mediated NF-?B pathway in gastric cancer.
Long non-coding RNAs (lncRNAs) have emerged as critical regulators in gastric cancer (GC). LncRNA expression microarray data indicate that KRT19P3 (Keratin 19 Pseudogene 3) is downregulated in GC samples. However, the expression pattern and molecular mechanism of KRT19P3 in GC have not been characterized. The present study confirmed the downregulation of KRT19P3 in GC tissues and cells. Decreased expression of ... [more]
Throughput
- Low Throughput
Additional Notes
- the protein corresponding to the bait gene, COPS7A, was IP'd (immunoprecipitated) and RNA corresponding to the hit gene, KRT19P3, was identified as an interacting partner by RT-qPCR (reverse transcription-quantitative polymerase chain reaction) and validated by gel electrophoresis
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KRT19P3 COPS7A | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | 2768032 |
Curated By
- BioGRID