BAIT

AGO2

EIF2C2, Q10
argonaute RISC catalytic component 2
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3?/?-catenin signaling pathway.

Liu Y, Jiang Q, Liu X, Lin X, Tang Z, Liu C, Zhou J, Zhao M, Li X, Cheng Z, Li L, Xie Y, Liu Z, Fang W

Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-related tumor. The role of EBV-encoding miR-BART22 is still unclear in NPC. This study aimed to identify the detailed mechanisms by which EBV-miR-BART22 functions as a tumor-promoting factor and evaluate the action of cinobufotalin in treating EBV-miR-BART22-overexpressing NPC cells.Using real-time PCR, western blotting, immunohistochemistry, and In situ hybridization, we detected the expression of ... [more]

EBioMedicine Oct. 01, 2019; 48();386-404 [Pubmed: 31594754]

Throughput

  • Low Throughput

Additional Notes

  • interaction between RNA that corresponds to the hit gene, MAP2K4, and protein that corresponds to the bait gene, Ago2, detected by co-IP (co-immunoprecipitation)

Curated By

  • BioGRID