Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Gamma1- and gamma2-syntrophins, two novel dystrophin-binding proteins localized in neuronal cells.

Piluso G, Mirabella M, Ricci E, Belsito A, Abbondanza C, Servidei S, Puca AA, Tonali P, Puca GA, Nigro V

Dystrophin is the scaffold of a protein complex, disrupted in inherited muscular dystrophies. At the last 3' terminus of the gene, a protein domain is encoded, where syntrophins are tightly bound. These are a family of cytoplasmic peripheral membrane proteins. Three genes have been described encoding one acidic (alpha1) and two basic (beta1 and beta2) proteins of approximately 57-60 kDa. ... [more]

J. Biol. Chem. May. 26, 2000; 275(21);15851-60 [Pubmed: 10747910]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNTA1 DTNA	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lyssand JS (2008)	Low	-	BioGRID	888713
DTNA SNTA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Sadoulet-Puccio HM (1997)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DTNA

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SNTA1