Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Surface expression of a glycolytic enzyme, alpha-enolase, recognized by autoantibodies in connective tissue disorders.

Moscato S, Pratesi F, Sabbatini A, Chimenti D, Scavuzzo M, Passatino R, Bombardieri S, Giallongo A, Migliorini P

In systemic autoimmune diseases, autoantibodies specific for alpha-enolase are detected more frequently in patients with active renal involvement. To analyze the properties of anti-alpha-enolase antibodies and the distribution of the enzyme in the cell, mouse monoclonal and polyclonal antibodies were obtained from mice immunized with a glutathione-S-transferase-alpha-enolase fusion protein. Anti-alpha-enolase antibodies were purified from patient sera on enolase from human ... [more]

Eur. J. Immunol. Dec. 01, 2000; 30(12);3575-84 [Pubmed: 11169399]

Throughput

  • Low Throughput

Curated By

  • BioGRID