BAIT

HAB1

F28P22.4, F28P22_4, homology to ABI1, AT1G72770
protein phosphatase 2C 16
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

PYL1

MZA15.21, MZA15_21, PYR1-like 1, RCAR12, regulatory components of ABA receptor 12, AT5G46790
abscisic acid receptor PYL1
GO Process (2)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Extensive signal integration by the phytohormone protein network.

Altmann M, Altmann S, Rodriguez PA, Weller B, Elorduy Vergara L, Palme J, Marin-de la Rosa N, Sauer M, Wenig M, Villaecija-Aguilar JA, Sales J, Lin CW, Pandiarajan R, Young V, Strobel A, Gross L, Carbonnel S, Kugler KG, Garcia-Molina A, Bassel GW, Falter C, Mayer KFX, Gutjahr C, Vlot AC, Grill E, Falter-Braun P

Plant hormones coordinate responses to environmental cues with developmental programs1, and are fundamental for stress resilience and agronomic yield2. The core signalling pathways underlying the effects of phytohormones have been elucidated by genetic screens and hypothesis-driven approaches, and extended by interactome studies of select pathways3. However, fundamental questions remain about how information from different pathways is integrated. Genetically, most phenotypes ... [more]

Nature Dec. 01, 2019; 583(7815);271-276 [Pubmed: 32612234]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PYL1 HAB1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
HAB1 PYL1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
PYL1 HAB1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID