BAIT

ABCD2

ABC39, ALDL1, ALDR, ALDRP
ATP-binding cassette, sub-family D (ALD), member 2
Mus musculus
PREY

ABCD1

ALDP, Ald, Aldgh, RP23-373N8.2
ATP-binding cassette, sub-family D (ALD), member 1
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Homo- and heterodimerization of peroxisomal ATP-binding cassette half-transporters.

Liu LX, Janvier K, Berteaux-Lecellier V, Cartier N, Benarous R, Aubourg P

Mammalian peroxisomal proteins adrenoleukodystrophy protein (ALDP), adrenoleukodystrophy-related protein (ALDRP), and 70-kDa peroxisomal protein (PMP70) belong to the superfamily of ATP-binding cassette (ABC) transporters. Unlike many ABC transporters that are single functional proteins with two related halves, ALDP, ALDRP, and PMP70 have the structure of ABC half-transporters. The dysfunction of ALDP is responsible for X-linked adrenoleukodystrophy (X-ALD), a neurodegenerative disorder in ... [more]

J. Biol. Chem. Nov. 12, 1999; 274(46);32738-43 [Pubmed: 10551832]

Throughput

  • Low Throughput

Curated By

  • BioGRID