SC
Gene Ontology Biological Process
- Malpighian tubule tip cell differentiation [IMP]
- central nervous system development [NAS, TAS]
- chaeta development [IMP]
- chaeta morphogenesis [IMP, NAS]
- dosage compensation [NAS]
- negative regulation of apoptotic process [IMP]
- nervous system development [IGI, TAS]
- neuroblast fate determination [NAS, TAS]
- peripheral nervous system development [IMP, NAS, TAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of mitotic cell cycle [TAS]
- regulation of transcription from RNA polymerase II promoter [ISS]
- regulation of transcription, DNA-templated [ISS, NAS]
- sensory organ development [IMP]
- sex determination [IGI, IMP, NAS, TAS]
- sex determination, establishment of X:A ratio [NAS]
- ventral cord development [NAS]
Gene Ontology Molecular Function- DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
- transcription factor binding [IGI]
- DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA, IPI]
- sequence-specific DNA binding transcription factor activity [ISS]
- transcription factor binding [IGI]
Gene Ontology Cellular Component
EMC
Gene Ontology Biological Process
- R3/R4 cell differentiation [IMP]
- R7 cell differentiation [IMP]
- R8 cell fate commitment [NAS]
- brain morphogenesis [IMP]
- cell proliferation [IGI, IMP]
- chaeta morphogenesis [TAS]
- chorion-containing eggshell pattern formation [IMP]
- compound eye cone cell differentiation [IMP]
- dorsal appendage formation [IMP]
- dorsal closure [IMP]
- head involution [IMP]
- imaginal disc-derived wing morphogenesis [IGI, IMP]
- imaginal disc-derived wing vein morphogenesis [IGI, IMP]
- inter-male aggressive behavior [IMP]
- lateral inhibition [IMP]
- locomotion involved in locomotory behavior [IMP]
- midgut development [IMP]
- negative regulation of DNA binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- peripheral nervous system development [IMP]
- photoreceptor cell fate commitment [IMP]
- progression of morphogenetic furrow involved in compound eye morphogenesis [IMP]
- sex determination [IGI]
- sex determination, primary response to X:A ratio [TAS]
- spermatid development [IMP]
- startle response [IMP]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Comprehensive Drosophila melanogaster Transcription Factor Interactome.
Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SC EMC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| SC EMC | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| SC EMC | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| EMC SC | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| EMC SC | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2773597 | |
| EMC SC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| EMC SC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| EMC SC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - |
Curated By
- BioGRID