BAIT

SHC1

SHC, SHCA, RP11-307C12.1
SHC (Src homology 2 domain containing) transforming protein 1
Homo sapiens
PREY

PTK2

FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK
protein tyrosine kinase 2
GO Process (42)
GO Function (8)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Focal adhesion kinase enhances signaling through the Shc/extracellular signal-regulated kinase pathway in anaplastic astrocytoma tumor biopsy samples.

Hecker TP, Grammer JR, Gillespie GY, Stewart J, Gladson CL

Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase that on activation generates signals that can modulate crucial cell functions, including cell proliferation, migration, and survival. In vitro, overexpression of FAK has been shown to promote cell proliferation by signaling through the Ras/mitogen-activated protein kinase cascade in several cell types. We have shown previously that overexpression of exogenous FAK lacking ... [more]

Cancer Res. May. 01, 2002; 62(9);2699-707 [Pubmed: 11980671]

Throughput

  • Low Throughput

Curated By

  • BioGRID