NC2ALPHA
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NC2BETA
Gene Ontology Biological Process
- chromatin remodeling [IDA]
- histone acetylation [IDA]
- negative regulation of RNA polymerase II transcriptional preinitiation complex assembly [NAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nucleosome mobilization [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
A Comprehensive Drosophila melanogaster Transcription Factor Interactome.
Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and ... [more]
Throughput
- Low Throughput
Additional Notes
- MITOMI
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NC2ALPHA NC2BETA | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 2773593 | |
| NC2ALPHA NC2BETA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| NC2ALPHA NC2BETA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID