UNC13B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RIMS1
Gene Ontology Biological Process
- calcium ion-dependent exocytosis [TAS]
- glutamate secretion [TAS]
- membrane fusion [NAS]
- neurotransmitter secretion [TAS]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of inhibitory postsynaptic membrane potential [ISS]
- protein complex assembly [IDA]
- regulated secretory pathway [NAS]
- regulation of catalytic activity [TAS]
- regulation of neurotransmitter secretion [TAS]
- secretion [NAS]
- synaptic transmission [TAS]
- synaptic vesicle exocytosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming.
Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca2+-triggered fusion are taking place in a highly coordinated manner. We show that the active zone components Munc13-1, an essential vesicle priming protein, and RIM1, a Rab3 effector with a putative role in vesicle tethering, interact functionally. Disruption of this ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RIMS1 UNC13B | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RIMS1 UNC13B | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| UNC13B RIMS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID