An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex.

Kuessel-Andermann P, El-Amraoui A, Safieddine S, Nouaille S, Perfettini I, Lecuit M, Cossart P, Wolfrum U, Petit C

Defects in myosin VIIA are responsible for deafness in the human and mouse. The role of this unconventional myosin in the sensory hair cells of the inner ear is not yet understood. Here we show that the C-terminal FERM domain of myosin VIIA binds to a novel transmembrane protein, vezatin, which we identified by a yeast two-hybrid screen. Vezatin is ... [more]

EMBO J. Nov. 15, 2000; 19(22);6020-9 [Pubmed: 11080149]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MYO7A VEZT	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Kuessel-Andermann P (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MYO7A

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin filament binding [IDA]calmodulin binding [IMP]microfilament motor activity [IDA]protein binding [IPI]spectrin binding [IDA]

Gene Ontology Cellular Component

VEZT