BAIT

EMC2

KIAA0103, TTC35
ER membrane protein complex subunit 2
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Homo sapiens
PREY

HSP90B1

ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1
heat shock protein 90kDa beta (Grp94), member 1
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics.

Savidis G, McDougall WM, Meraner P, Perreira JM, Portmann JM, Trincucci G, John SP, Aker AM, Renzette N, Robbins DR, Guo Z, Green S, Kowalik TF, Brass AL

The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we completed orthologous functional genomic screens using RNAi and CRISPR/Cas9 approaches. The screens recovered the ZIKV entry factor AXL as well as multiple host factors involved in endocytosis (RAB5C and RABGEF), heparin sulfation ... [more]

Cell Rep Dec. 28, 2015; 16(1);232-246 [Pubmed: 27342126]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EMC2 HSP90B1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2708678

Curated By

  • BioGRID