BAIT
CASQ2
PDIB2
calsequestrin 2 (cardiac muscle)
GO Process (17)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
- Purkinje myocyte to ventricular cardiac muscle cell signaling [NAS]
- cardiac muscle contraction [IMP]
- cellular response to caffeine [IMP]
- detection of calcium ion [TAS]
- ion transmembrane transport [TAS]
- negative regulation of potassium ion transmembrane transporter activity [ISS]
- negative regulation of potassium ion transport [ISS]
- negative regulation of ryanodine-sensitive calcium-release channel activity [IDA, ISS]
- protein polymerization [IDA]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IMP]
- regulation of cell communication by electrical coupling [IMP]
- regulation of heart rate [IMP]
- regulation of membrane repolarization [ISS]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISS]
- sequestering of calcium ion [IDA, IMP]
- striated muscle contraction [TAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
PLEC
EBS1, EBSO, HD1, LGMD2Q, PCN, PLEC1, PLEC1b, PLTN
plectin
GO Process (5)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): lysates from unfractionated cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using TX100-soluble or TX100-insoluble fractions
Curated By
- BioGRID