BAIT
RAD51D
BROVCA4, R51H3, RAD51L3, TRAD
RAD51 paralog D
GO Process (6)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SPTBN4
QV, SPNB4, SPTBN3
spectrin, beta, non-erythrocytic 4
GO Process (14)
GO Function (6)
GO Component (14)
Gene Ontology Biological Process
- adult walking behavior [ISS]
- axon guidance [TAS]
- axonogenesis [ISS]
- cardiac conduction [ISS]
- central nervous system projection neuron axonogenesis [ISS]
- clustering of voltage-gated sodium channels [ISS]
- cytoskeletal anchoring at plasma membrane [TAS]
- establishment of protein localization to plasma membrane [ISS]
- negative regulation of heart rate [ISS]
- regulation of peptidyl-serine phosphorylation [ISS]
- regulation of sodium ion transport [ISS]
- sensory perception of sound [ISS]
- transmission of nerve impulse [ISS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- PML body [IDA, ISS]
- adherens junction [ISS]
- axon hillock [ISS]
- axon initial segment [ISS]
- cell body fiber [ISS]
- cytoplasm [IDA, ISS]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- membrane [IDA]
- neuronal cell body [ISS]
- node of Ranvier [ISS]
- nuclear matrix [IDA, ISS]
- plasma membrane [ISS]
- spectrin [IDA, ISS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): lysates from unfractionated cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using TX100-soluble or TX100-insoluble fractions
Curated By
- BioGRID