STAT1
Gene Ontology Biological Process
- JAK-STAT cascade [IDA]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- apoptotic process [ISS]
- blood circulation [ISS]
- cellular response to interferon-beta [IMP]
- cytokine-mediated signaling pathway [TAS]
- endothelial cell migration [IMP]
- interferon-gamma-mediated signaling pathway [IDA, ISS, TAS]
- metanephric mesenchymal cell differentiation [ISS]
- metanephric mesenchymal cell proliferation involved in metanephros development [ISS]
- negative regulation by virus of viral protein levels in host cell [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- negative regulation of angiogenesis [IMP]
- negative regulation of endothelial cell proliferation [IMP]
- negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis [ISS]
- negative regulation of metanephric nephron tubule epithelial cell differentiation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of mesenchymal cell proliferation [ISS]
- positive regulation of smooth muscle cell proliferation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of apoptotic process [TAS]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of type I interferon-mediated signaling pathway [TAS]
- renal tubule development [IMP]
- response to cAMP [ISS]
- response to cytokine [ISS]
- response to peptide hormone [ISS]
- transcription from RNA polymerase II promoter [IDA]
- tumor necrosis factor-mediated signaling pathway [IDA]
- type I interferon signaling pathway [ISS, TAS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- double-stranded DNA binding [IDA]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- tumor necrosis factor receptor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- double-stranded DNA binding [IDA]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- tumor necrosis factor receptor binding [IPI]
PTK2
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- angiogenesis [IBA, TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell motility [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cytoskeleton organization [IBA]
- embryo development [TAS]
- ephrin receptor signaling pathway [IDA]
- epidermal growth factor receptor signaling pathway [IBA]
- establishment of cell polarity [TAS]
- growth hormone receptor signaling pathway [IDA]
- heart morphogenesis [TAS]
- innate immune response [IBA, TAS]
- integrin-mediated signaling pathway [IDA, IMP, TAS]
- negative regulation of anoikis [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell-cell adhesion [IDA]
- netrin-activated signaling pathway [TAS]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA]
- placenta development [TAS]
- platelet activation [TAS]
- positive regulation of cell migration [IDA]
- positive regulation of cell proliferation [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [TAS]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- protein autophosphorylation [IDA]
- regulation of Rho GTPase activity [TAS]
- regulation of cell adhesion mediated by integrin [IDA]
- regulation of cell proliferation [IMP]
- regulation of cell shape [IMP]
- regulation of cytoskeleton organization [TAS]
- regulation of endothelial cell migration [TAS]
- regulation of focal adhesion assembly [TAS]
- regulation of osteoblast differentiation [IMP]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Focal adhesion kinase activates Stat1 in integrin-mediated cell migration and adhesion.
Recent studies suggest that focal adhesion kinase (FAK) is important for cell migration. We now suggest a mechanism by which FAK activates the signal transducer and activator of transcription (STAT) pathway, regulating cell adhesion and migration. In particular, we observe that FAK is capable of activating Stat1, but not Stat3. Co-immunoprecipitation and in vitro binding assays demonstrate that Stat1 is ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PTK2 STAT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STAT1 PTK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STAT1 PTK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PTK2 STAT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STAT1 PTK2 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | High | - | BioGRID | 1504989 |
Curated By
- BioGRID