BAIT
NCKAP1L
HEM1
NCK-associated protein 1-like
GO Process (31)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- B cell homeostasis [ISS]
- B cell receptor signaling pathway [IMP]
- T cell homeostasis [ISS]
- actin polymerization-dependent cell motility [IMP]
- chemotaxis [IDA]
- cortical actin cytoskeleton organization [IMP]
- erythrocyte development [IEP]
- maintenance of cell polarity [IMP]
- myeloid cell homeostasis [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of interleukin-17 production [ISS]
- negative regulation of interleukin-6 production [ISS]
- negative regulation of myosin-light-chain-phosphatase activity [IMP]
- neutrophil chemotaxis [IDA]
- positive regulation of B cell differentiation [ISS]
- positive regulation of B cell proliferation [IMP]
- positive regulation of CD4-positive, alpha-beta T cell differentiation [ISS]
- positive regulation of CD8-positive, alpha-beta T cell differentiation [ISS]
- positive regulation of Rac GTPase activity [IMP]
- positive regulation of T cell proliferation [ISS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of cell adhesion mediated by integrin [ISS]
- positive regulation of erythrocyte differentiation [ISS]
- positive regulation of gamma-delta T cell differentiation [ISS]
- positive regulation of lymphocyte differentiation [ISS]
- positive regulation of neutrophil chemotaxis [ISS]
- positive regulation of phagocytosis, engulfment [ISS]
- positive regulation of phosphorylation [IMP]
- positive regulation of protein kinase activity [IMP]
- protein complex assembly [ISS]
- response to drug [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
HIST3H3
H3.4, H3/g, H3FT, H3t
histone cluster 3, H3
GO Process (1)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): lysates from unfractionated cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using TX100-soluble or TX100-insoluble fractions
Curated By
- BioGRID