BAIT
GABRD
EIG10, EJM7, GEFSP5
gamma-aminobutyric acid (GABA) A receptor, delta
GO Process (2)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB13
GIG4
RAB13, member RAS oncogene family
GO Process (15)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISS]
- cortical actin cytoskeleton organization [IBA, ISS]
- endocytic recycling [IMP]
- endosomal transport [IMP]
- endothelial cell chemotaxis [ISS]
- establishment of Sertoli cell barrier [ISS]
- establishment of protein localization to plasma membrane [IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- neuron projection development [IMP]
- protein kinase A signaling [IMP]
- tight junction assembly [IMP]
- trans-Golgi network to recycling endosome transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- endocytic vesicle [IDA]
- extracellular vesicular exosome [IDA]
- insulin-responsive compartment [ISS]
- lamellipodium [ISS]
- lateral plasma membrane [IDA]
- neuron projection [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tight junction [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-insoluble fractions
Curated By
- BioGRID