An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

ZIP3, a new splice variant of the PKC-zeta-interacting protein family, binds to GABAC receptors, PKC-zeta, and Kv beta 2.

Croci C, Braendstatter JH, Enz R

The correct targeting of modifying enzymes to ion channels and neurotransmitter receptors represents an important biological mechanism to control neuronal excitability. The recent cloning of protein kinase C-zeta interacting proteins (ZIP1, ZIP2) identified new scaffolds linking the atypical protein kinase PKC-zeta to target proteins. GABA(C) receptors are composed of three rho subunits (rho 1-3) that are highly expressed in the ... [more]

J. Biol. Chem. Feb. 21, 2003; 278(8);6128-35 [Pubmed: 12431995]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SQSTM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

K63-linked polyubiquitin binding [ISO]SH2 domain binding [ISO, ISS]identical protein binding [ISO]protein binding [IPI]protein homodimerization activity [IDA]protein kinase C binding [IPI, ISO, ISS]protein kinase binding [ISO]ubiquitin binding [IBA, ISO, ISS]

Gene Ontology Cellular Component

KCNAB2