BAIT
CXCL12
IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
chemokine (C-X-C motif) ligand 12
GO Process (17)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- blood circulation [TAS]
- cell adhesion [TAS]
- cell chemotaxis [IDA]
- cellular calcium ion homeostasis [TAS]
- chemokine-mediated signaling pathway [IDA]
- chemotaxis [TAS]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- negative regulation of leukocyte apoptotic process [IDA]
- negative regulation of leukocyte tethering or rolling [IDA]
- positive regulation of T cell migration [IDA]
- positive regulation of calcium ion import [TAS]
- positive regulation of cell adhesion [IDA]
- positive regulation of monocyte chemotaxis [IDA]
- regulation of actin polymerization or depolymerization [TAS]
- response to virus [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PDIA6
ERP5, P5, TXNDC7
protein disulfide isomerase family A, member 6
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-insoluble fractions
Curated By
- BioGRID