BAIT
MED1
CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
mediator complex subunit 1
GO Process (32)
GO Function (20)
GO Component (5)
Gene Ontology Biological Process
- ERK1 and ERK2 cascade [IDA]
- androgen biosynthetic process [IMP]
- androgen receptor signaling pathway [IDA]
- angiogenesis [ISS]
- cell morphogenesis [IMP]
- cellular lipid metabolic process [TAS]
- cellular response to epidermal growth factor stimulus [IDA]
- cellular response to steroid hormone stimulus [IDA]
- cellular response to thyroid hormone stimulus [IDA]
- erythrocyte development [ISS]
- fat cell differentiation [IDA]
- gene expression [TAS]
- intracellular steroid hormone receptor signaling pathway [IDA]
- keratinocyte differentiation [IMP]
- lens development in camera-type eye [ISS]
- mRNA transcription from RNA polymerase II promoter [ISS]
- megakaryocyte development [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of keratinocyte proliferation [IMP]
- negative regulation of neuron differentiation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of gene expression [IDA, IMP]
- positive regulation of keratinocyte differentiation [IMP]
- positive regulation of receptor activity [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of RNA biosynthetic process [IMP]
- regulation of cell cycle [NAS]
- regulation of transcription from RNA polymerase I promoter [IDA]
- small molecule metabolic process [TAS]
- thyroid hormone mediated signaling pathway [IMP]
- transcription initiation from RNA polymerase II promoter [IDA, TAS]
Gene Ontology Molecular Function- LBD domain binding [IPI]
- RNA polymerase II transcription cofactor activity [IDA]
- chromatin binding [IMP]
- core promoter binding [IDA]
- estrogen receptor binding [IPI]
- ligand-dependent nuclear receptor binding [IDA, IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP, NAS]
- mediator complex binding [IDA]
- nuclear hormone receptor binding [IPI]
- peroxisome proliferator activated receptor binding [IPI]
- protein binding [IPI]
- receptor activity [IDA]
- retinoic acid receptor binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- thyroid hormone receptor binding [IDA, IPI]
- thyroid hormone receptor coactivator activity [IMP]
- transcription coactivator activity [IDA, IMP]
- transcription cofactor activity [IDA]
- transcription factor binding [IPI]
- vitamin D receptor binding [IPI, NAS, TAS]
- LBD domain binding [IPI]
- RNA polymerase II transcription cofactor activity [IDA]
- chromatin binding [IMP]
- core promoter binding [IDA]
- estrogen receptor binding [IPI]
- ligand-dependent nuclear receptor binding [IDA, IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP, NAS]
- mediator complex binding [IDA]
- nuclear hormone receptor binding [IPI]
- peroxisome proliferator activated receptor binding [IPI]
- protein binding [IPI]
- receptor activity [IDA]
- retinoic acid receptor binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- thyroid hormone receptor binding [IDA, IPI]
- thyroid hormone receptor coactivator activity [IMP]
- transcription coactivator activity [IDA, IMP]
- transcription cofactor activity [IDA]
- transcription factor binding [IPI]
- vitamin D receptor binding [IPI, NAS, TAS]
Homo sapiens
PREY
LRRC59
p34, PRO1855
leucine rich repeat containing 59
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-insoluble fractions
Curated By
- BioGRID