An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Human mitochondrial import receptor, Tom20p. Use of glutathione to reveal specific interactions between Tom20-glutathione S-transferase and mitochondrial precursor proteins.

Schleiff E, Shore GC, Goping IS

The cytosolic domain of the human mitochondrial protein import receptor, hTom20, has been expressed as a fusion protein with glutathione S-transferase (GST) in bacteria and the purified protein immobilized on Sepharose beads. To discriminate between specific binding of precursor proteins with the receptor and non-specific binding, precursors were recovered as a complex with GST-hTom20 following competitive elution from the beads ... [more]

FEBS Lett. Mar. 10, 1997; 404(2);314-8 [Pubmed: 9119086]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TOMM20

Gene Ontology Biological Process

Gene Ontology Molecular Function

P-P-bond-hydrolysis-driven protein transmembrane transporter activity [IDA]protein binding [IPI]unfolded protein binding [IDA]

Gene Ontology Cellular Component

UCP1