BAIT
SEC31A
ABP125, ABP130, HSPC334, SEC31L1, HSPC275
SEC31 homolog A (S. cerevisiae)
GO Process (11)
GO Function (2)
GO Component (12)
Gene Ontology Biological Process
- COPII vesicle coating [TAS]
- ER to Golgi vesicle-mediated transport [NAS, TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- membrane organization [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- response to calcium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COPII vesicle coat [NAS]
- ER to Golgi transport vesicle [IDA]
- ER to Golgi transport vesicle membrane [TAS]
- Golgi membrane [TAS]
- cytoplasm [IDA]
- cytosol [TAS]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum exit site [IMP]
- endoplasmic reticulum membrane [TAS]
- intracellular membrane-bounded organelle [IDA]
- perinuclear region of cytoplasm [IDA]
- vesicle coat [IDA]
Homo sapiens
PREY
LUZP1
LUZP, RP1-184J9.3
leucine zipper protein 1
GO Process (0)
GO Function (0)
GO Component (2)
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-insoluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-soluble fractions
Curated By
- BioGRID