BAIT
RPLP2
D11S2243E, LP2, P2, RPP2
ribosomal protein, large, P2
GO Process (13)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PKHD1
ARPKD, FCYT, TIGM1
polycystic kidney and hepatic disease 1 (autosomal recessive)
GO Process (13)
GO Function (2)
GO Component (9)
Gene Ontology Biological Process
- cellular calcium ion homeostasis [IMP]
- cilium assembly [ISS]
- homeostatic process [NAS]
- kidney development [ISS]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cellular component movement [ISS]
- negative regulation of protein kinase B signaling [IMP]
- positive regulation of cell proliferation [IMP]
- regulation of ERK1 and ERK2 cascade [IMP]
- regulation of TOR signaling [IMP]
- regulation of centrosome duplication [IMP]
- single organismal cell-cell adhesion [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-insoluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-soluble fractions
Curated By
- BioGRID