BAIT

PLCG1

NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, RP3-511B24.2
phospholipase C, gamma 1
Homo sapiens
PREY

SH3BP2

3BP-2, 3BP2, CRBM, CRPM, RES4-23
SH3-domain binding protein 2
GO Process (2)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

Regulation of NK cell-mediated cytotoxicity by the adaptor protein 3BP2.

Jevremovic D, Billadeau DD, Schoon RA, Dick CJ, Leibson PJ

Stimulation of lymphocytes through multichain immune recognition receptors activates multiple signaling pathways. Adaptor proteins play an important role in integrating these pathways by their ability to simultaneously bind multiple signaling components. Recently, the 3BP2 adaptor protein has been shown to positively regulate the transcriptional activity of T cells. However, the mechanisms by which signaling components are involved in this regulation ... [more]

J. Immunol. Jun. 15, 2001; 166(12);7219-28 [Pubmed: 11390470]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SH3BP2 PLCG1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID