An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Human leptin forms complexes with alpha 2-macroglobulin which are recognized by the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein.

Birkenmeier G, Kaempfer I, Kratzsch J, Schellenberger W

OBJECTIVE: To identify binding proteins of leptin in human plasma. METHODS: Binding was evaluated by electrophoresis, size exclusion chromatography (SEC), Western blotting, and radioisotope labeling. Quantification of leptin and the different forms of alpha2-macroglobulin (alpha2-M) was performed by ELISA. RESULTS: Leptin interacts with the proteinase inhibitor, alpha2-M. 125I-labeled leptin specifically binds to the transformed inhibitor, which arises by reaction with ... [more]

Eur. J. Endocrinol. Aug. 01, 1998; 139(2);224-30 [Pubmed: 9724081]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

A2M

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium-dependent protein binding [IPI]enzyme binding [IPI]growth factor binding [IDA]interleukin-1 binding [IDA]interleukin-8 binding [IPI]protease binding [IPI]protein binding [IPI]receptor binding [IMP]serine-type endopeptidase inhibitor activity [IDA]tumor necrosis factor binding [IDA]

Gene Ontology Cellular Component

LEP