BAIT
DNA2
DNA2L, hDNA2, RP11-9E13.1
DNA replication helicase/nuclease 2
GO Process (17)
GO Function (10)
GO Component (4)
Gene Ontology Biological Process
- ATP catabolic process [IDA, TAS]
- DNA catabolic process, endonucleolytic [IDA]
- DNA double-strand break processing [IDA]
- DNA duplex unwinding [IDA]
- DNA replication [IMP]
- DNA replication checkpoint [IMP]
- DNA replication, Okazaki fragment processing [IDA]
- DNA replication, removal of RNA primer [IDA]
- DNA strand elongation involved in DNA replication [TAS]
- base-excision repair [IDA]
- mitochondrial DNA repair [IDA]
- mitochondrial DNA replication [IDA]
- mitotic cell cycle [TAS]
- positive regulation of DNA replication [IDA]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
Gene Ontology Molecular Function- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
PLEC
EBS1, EBSO, HD1, LGMD2Q, PCN, PLEC1, PLEC1b, PLTN
plectin
GO Process (5)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-insoluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-soluble fractions
Curated By
- BioGRID