Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit.

Boldyreff B, Issinger OG

In a search for protein kinase CK2 beta subunit binding proteins using the two-hybrid system, more than 1000 positive clones were isolated. Beside clones for the alpha' and beta subunit of CK2, there were clones coding for a so far unknown protein, whose partial cDNA sequence was already deposited in the EMBL database under the accession numbers R08806 and Z17360, ... [more]

FEBS Lett. Feb. 17, 1997; 403(2);197-9 [Pubmed: 9042965]

Throughput

  • Low Throughput

Curated By

  • BioGRID