BAIT

GOLGB1

GCP, GCP372, GOLIM1
golgin B1
GO Process (1)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ACBD3

GCP60, GOCAP1, GOLPH1, PAP7
acyl-CoA binding domain containing 3
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin.

Sohda M, Misumi Y, Yamamoto A, Yano A, Nakamura N, Ikehara Y

We demonstrated previously that the integral membrane protein giantin has the Golgi localization signal at the COOH-terminal cytoplasmic domain (Misumi, Y., Sohda, M., Tashiro, A., Sato, H., and Ikehara, Y. (2001) J. Biol. Chem. 276, 6867-6873). In the present study, using this domain as bait in the yeast two-hybrid screening system, we identified a novel protein interacting with giantin. The ... [more]

J. Biol. Chem. Nov. 30, 2001; 276(48);45298-306 [Pubmed: 11590181]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GOLGB1 ACBD3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID