BAIT
GOLGB1
GCP, GCP372, GOLIM1
golgin B1
GO Process (1)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ACBD3
GCP60, GOCAP1, GOLPH1, PAP7
acyl-CoA binding domain containing 3
GO Process (0)
GO Function (1)
GO Component (2)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Far Western
An interaction is detected between a protein immobilized on a membrane and a purified protein probe.
Publication
Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin.
We demonstrated previously that the integral membrane protein giantin has the Golgi localization signal at the COOH-terminal cytoplasmic domain (Misumi, Y., Sohda, M., Tashiro, A., Sato, H., and Ikehara, Y. (2001) J. Biol. Chem. 276, 6867-6873). In the present study, using this domain as bait in the yeast two-hybrid screening system, we identified a novel protein interacting with giantin. The ... [more]
J. Biol. Chem. Nov. 30, 2001; 276(48);45298-306 [Pubmed: 11590181]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GOLGB1 ACBD3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID