BAIT
HIST3H3
H3.4, H3/g, H3FT, H3t
histone cluster 3, H3
GO Process (1)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAPGEF2
CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, Rap-GEP, nRap GEP
Rap guanine nucleotide exchange factor (GEF) 2
GO Process (35)
GO Function (12)
GO Component (11)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- MAPK cascade [NAS]
- Rap protein signal transduction [IMP]
- adenylate cyclase-activating adrenergic receptor signaling pathway [IDA]
- blood vessel development [ISS]
- brain-derived neurotrophic factor receptor signaling pathway [ISS]
- cAMP-mediated signaling [IDA, NAS]
- cellular response to cAMP [IDA]
- cellular response to cGMP [IDA]
- cellular response to nerve growth factor stimulus [ISS]
- establishment of endothelial barrier [IMP]
- forebrain neuron development [ISS]
- intracellular signal transduction [TAS]
- negative regulation of cell proliferation [IDA]
- negative regulation of dendrite morphogenesis [IDA]
- negative regulation of melanin biosynthetic process [ISS]
- nerve growth factor signaling pathway [ISS]
- neuron migration [ISS]
- neuron projection development [IDA]
- neuropeptide signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of Rap GTPase activity [IDA, IMP]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of cAMP-dependent protein kinase activity [IDA]
- positive regulation of cAMP-mediated signaling [IDA]
- positive regulation of dendritic cell apoptotic process [IDA]
- positive regulation of neuron migration [ISS]
- positive regulation of neuron projection development [ISS]
- positive regulation of protein binding [ISS]
- positive regulation of protein kinase activity [IDA]
- positive regulation of vasculogenesis [ISS]
- regulation of cell junction assembly [IMP]
- regulation of synaptic plasticity [ISS]
- small GTPase mediated signal transduction [TAS]
- ventricular system development [ISS]
Gene Ontology Molecular Function- PDZ domain binding [IDA]
- Rap GTPase activator activity [IDA]
- Rap guanyl-nucleotide exchange factor activity [IDA, IMP]
- Ras guanyl-nucleotide exchange factor activity [IDA]
- WW domain binding [IDA]
- beta-1 adrenergic receptor binding [IDA]
- cAMP binding [IDA]
- cGMP binding [IDA]
- calcium ion binding [NAS]
- diacylglycerol binding [NAS]
- protein binding [IPI]
- signal transducer activity [TAS]
- PDZ domain binding [IDA]
- Rap GTPase activator activity [IDA]
- Rap guanyl-nucleotide exchange factor activity [IDA, IMP]
- Ras guanyl-nucleotide exchange factor activity [IDA]
- WW domain binding [IDA]
- beta-1 adrenergic receptor binding [IDA]
- cAMP binding [IDA]
- cGMP binding [IDA]
- calcium ion binding [NAS]
- diacylglycerol binding [NAS]
- protein binding [IPI]
- signal transducer activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-insoluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-soluble fractions
Curated By
- BioGRID