BAIT
GOT1
ASTQTL1, GIG18, cAspAT, cCAT
glutamic-oxaloacetic transaminase 1, soluble
GO Process (16)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- 2-oxoglutarate metabolic process [ISS]
- L-methionine biosynthetic process from methylthioadenosine [TAS]
- aspartate catabolic process [IDA]
- aspartate metabolic process [ISS]
- carbohydrate metabolic process [TAS]
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- cellular response to insulin stimulus [IEP]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glutamate metabolic process [ISS]
- glycerol biosynthetic process [ISS]
- polyamine metabolic process [TAS]
- response to glucocorticoid [IEP]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
RPL6
L6, SHUJUN-2, TAXREB107, TXREB1
ribosomal protein L6
GO Process (14)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- regulation of transcription, DNA-templated [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): unfractionated cells or TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in a parallel experiment using TX100-insoluble fractions
Curated By
- BioGRID