Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The visualization by affinity electrophoresis of a specific association between the consecutive citric acid cycle enzymes fumarase and malate dehydrogenase.

Beeckmans S, Van Driessche E, Kanarek L

Evidence is growing that the citric acid cycle, like many other metabolic pathways, might exist in vivo as a more or less tightly organized multi-enzyme cluster. The term 'metabolon' [Robinson, J. B. & Srere, P. A. (1985) J. Biol. Chem. 260, 10800-10805] was recently introduced to describe such a complex of sequential metabolic enzymes. We adopted the technique of affinity ... [more]

Eur. J. Biochem. Aug. 01, 1989; 183(2);449-54 [Pubmed: 2759092]

Throughput

  • Low Throughput

Curated By

  • BioGRID